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1.5 kg paddy straw should be immersed in the water kept in the bucket . The straw should remain soaked in the water for 8 to 12 hours, so that all dirt is removed, and it becomes black in colour. The straw should then be rinsed in clean water.

After this the straw should be either immersed in boiling water for some time, or boiling water should be poured on the straw and drained after five minutes. The high temperature of the water will kill all the bacteria and fungus in the straw which could result in decay of the straw..

The cleaned straw will be then filled in plastic bags, in layers of size 6 inches by 4 inches, and the mushroom spawn will be applied on each straw layer, along the edge, which allows the mushrooms to be easily removed. The remaining spawn can be used to cover the top surface of the straw in the plastic bag. Rubber bands are used to cover the top of the plastic bag, which is then kept in a semi dark place, away from direct sunlight, with little ventilation.

In two to three weeks, the oyster mushrooms will be visible on the straw, and the plastic bag can be exposed to more light. It is advisable to spray water on the straw in the plastic bag, till all mushrooms grow to their full size. Precautions should be taken to ensure that no flies or mosquitoes or other insects, live like parasites on the mushroom

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